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Introduction to the Biological Buffer DIPSO 68399-80-4
Category : Other Chemicals/Others
CAS NO : 68399-80-4 EC NO : 269-992-2 MF : C7H17NO6S MW : 243.278 Specification : White Powder Packing : 500g/bottle Product description : The biological buffering agent DIPSO, also known as 3- [N, N-di (hydroxyethyl) amino] -2-hydroxypropanesulfonic acid, has a CAS number of 68399-80-4 and a white crystalline powder appearance. It is a zwitterionic buffering agent. At room temperature, the pH value of 0.1M DIPSO powder in water ranges from 4.0 to 5.5, making it an acidic liquid; The acidity coefficient pKa has a value of 7.6, and the pH value range of acid-base indicator discoloration is between 7.0 and 8.2. It is widely used in scientific research and chemical engineering fields. 1. Advantages of DIPSO As a member of the amphoteric ion buffer, DIPSO has improved its water solubility, chemical stability, and compatibility with other biological agents compared to traditional phosphate buffers. In addition, DIPSO is resistant to enzymatic and non enzymatic degradation, and is less affected by medium concentration, temperature, and ion composition. Not only does it have a low cytotoxic effect on cells, but the pKa value is similar to the pH value of organisms and has strong compatibility with biological systems. 2. Application of DIPSO DIPSO has a wide range of uses and is a commonly used buffer in biochemical separation and purification analysis, used to stabilize the pH value of the liquid required for experiments; It can also be used in electrophoresis experiments to provide the required ions and ensure the stability and repeatability of the migration process; It can also be combined with other buffering agents such as HEPES, MOPS, etc. to form new buffering agents, expanding the pH high activity range of the buffer and thereby reducing the required buffering agent concentration. Precautions for use The original shape of DIPSO is white crystalline powder, which needs to be prepared into a solution in a certain proportion when using. Protective tools should be worn during operation to avoid direct contact with human skin or eyes, ears, and nose; The aqueous solution of DIPSO is transparent and does not differ significantly from water to the naked eye. Therefore, the prepared DIPSO solution should be labeled in a timely manner to distinguish it. In addition, when storing DIPSO, it is advisable to seal it in a dry, ventilated, and light proof environment. DIPSO buffer is currently not commonly available on the market. If you want to purchase it, please contact Hubei Xindesheng Material Technology Co., Ltd. The DIPSO produced by Desheng has high purity and good water solubility; The production process is stable, there are small differences between batches, and there is a professional research and development team to ensure product quality! Uses : Biological buffer Synonyms : DIPSO;3-[bis(2-hydroxyethyl)amino]-2-hydroxypropane-1-sulfonic acid;(2S)-3-[bis(2-hydroxyethyl)ammonio]-2-hydroxypropane-1-sulfonate;(2R)-3-[bis(2-hydroxyethyl)ammonio]-2-hydroxypropane-1-sulfonate;3-[N,N-Bis(2-hydroxyethyl)amino]-2-hydroxy-1-propanesulfonic acid; Molecular Structure : 
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Introduction to the Biological Buffer DIPSO